Anti-beta-Arrestin 1(Ser412), ALEXA Fluor 594
- Size
- 100 microliters
- Catalog no.
- GENTObs-3048R-A594
- Price
- 516 EUR
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Subcellular locations
N/A
Concentration
1ug per 1ul
Excitation emission
590nm/617nm
Modification
Phosphorylation
Conjugated
Alexa conjugate 1
Conjugated with
ALEXA FLUOR® 594
Applications with corresponding dilutions
IF(IHC-P)(1:50-200)
Clonality
Polyclonal Antibody
Target Protein/Peptide
beta-Arrestin 1Ser412
Clone
Polyclonal Antibodies
Purification method
Purified by Protein A.
Type
Conjugated Primary Antibody
Other name
Anti-beta-Arrestin 1(Ser412)
Conjugation
Alexa Fluor,ALEXA FLUOR® 594
Host organism
Rabbit (Oryctolagus cuniculus)
Also known as
beta-Arrestin 1(Ser412) Antibody
Properties
For facs or microscopy Alexa 1 conjugate.
Specificity
This antibody reacts specifically with beta-Arrestin 1(Ser412)
Cross reactive species
Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)
Storage
Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
Antigen Source
KLH conjugated synthetic phosphopeptide derived from human beta-Arrestin 1 around the phosphorylation site of Ser412
Synonyms
beta Arrestin 1 phospho S412; p-beta Arrestin 1 phospho S412; ARB 1; ARB1; ARR 1; ARR1; ARRB 1; ARRB1; Arrestin beta 1.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Cross Reactive Species details
No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
Advisory
Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
Background information
Beta Arrestin 1 is a member of a family of proteins that are widely expressed but especially abundant in the central nervous system. Serving as an adaptor or scaffold molecule, beta Arrestin 1 is essential for mitogenic signaling. It mediates agonist dependent desensitization and internalization of G protein coupled receptors (GPCRs, e.g., beta 2 adrenergic receptor). After binding to their ligand and interacting with heterotrimeric G proteins, GPCRs are phosphorylated by G protein receptor kinases (GRKs) on serine residues. Beta Arrestin 1 has important roles in the cytoplasm and at the plasma membrane in the desensitization and internalization of G protein coupled receptors (GPCRs) and is increasingly appreciated to play an important role in the endocytosis and signaling of GPCRs. Beta Arrestin 1 in the cytosol is phosphorylated by ERK1 and 2 on serine 412 in a negative feedback mechanism and binds to the phosphorylated receptors at the plasma membrane. Serine 412 is then dephosphorylated and the GPCRs are internalized, leading to activation of the Ras, Raf, ERK1 and 2 signaling pathway.