Anti-beta-Arrestin 1(Ser412), ALEXA Fluor 594

Anti-beta-Arrestin 1(Ser412), ALEXA Fluor 594
Size
100 microliters
Catalog no.
GENTObs-3048R-A594
Price
516 EUR
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Subcellular locations

N/A

Gene ID

408

Swiss Prot

N/A

Modification site

Ser412

Applications

IF(IHC-P)

Concentration

1ug per 1ul

Excitation emission

590nm/617nm

Modification

Phosphorylation

Conjugated

Alexa conjugate 1

Conjugated with

ALEXA FLUOR® 594

Applications with corresponding dilutions

IF(IHC-P)(1:50-200)

Clonality

Polyclonal Antibody

Target Protein/Peptide

beta-Arrestin 1Ser412

Clone

Polyclonal Antibodies

Purification method

Purified by Protein A.

Type

Conjugated Primary Antibody

Other name

Anti-beta-Arrestin 1(Ser412)

Conjugation

Alexa Fluor,ALEXA FLUOR® 594

Host organism

Rabbit (Oryctolagus cuniculus)

Also known as

beta-Arrestin 1(Ser412) Antibody

Properties

For facs or microscopy Alexa 1 conjugate.

Specificity

This antibody reacts specifically with beta-Arrestin 1(Ser412)

Cross reactive species

Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)

Storage

Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.

Antigen Source

KLH conjugated synthetic phosphopeptide derived from human beta-Arrestin 1 around the phosphorylation site of Ser412

Synonyms

beta Arrestin 1 phospho S412; p-beta Arrestin 1 phospho S412; ARB 1; ARB1; ARR 1; ARR1; ARRB 1; ARRB1; Arrestin beta 1.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Cross Reactive Species details

No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.

Advisory

Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.

Background information

Beta Arrestin 1 is a member of a family of proteins that are widely expressed but especially abundant in the central nervous system. Serving as an adaptor or scaffold molecule, beta Arrestin 1 is essential for mitogenic signaling. It mediates agonist dependent desensitization and internalization of G protein coupled receptors (GPCRs, e.g., beta 2 adrenergic receptor). After binding to their ligand and interacting with heterotrimeric G proteins, GPCRs are phosphorylated by G protein receptor kinases (GRKs) on serine residues. Beta Arrestin 1 has important roles in the cytoplasm and at the plasma membrane in the desensitization and internalization of G protein coupled receptors (GPCRs) and is increasingly appreciated to play an important role in the endocytosis and signaling of GPCRs. Beta Arrestin 1 in the cytosol is phosphorylated by ERK1 and 2 on serine 412 in a negative feedback mechanism and binds to the phosphorylated receptors at the plasma membrane. Serine 412 is then dephosphorylated and the GPCRs are internalized, leading to activation of the Ras, Raf, ERK1 and 2 signaling pathway.