beta-Arrestin 1(Ser412) Antibody, HRP Conjugated

beta-Arrestin 1(Ser412) Antibody, HRP Conjugated
Size
0.1ml
Catalog no.
bs-3048R-HRP
Price
368 EUR
Buy

Gene ID number

408

Conjugation

HRP

Excitation Emission

N/A

Modification site

Ser412

Tested applications

WB, IHC-P

French translation

anticorps

Clonality

Polyclonal

Concentration

1ug per 1ul

Additional conjugation

HRP Conjugated

Modification

Phosphorylation

Crossreactivity

Human, Mouse, Rat

Clone

Polyclonal antibody

Target Antigen

beta-Arrestin 1Ser412

Category

Conjugated Primary Antibodies

Host Organism

Rabbit (Oryctolagus cuniculus)

Recommended dilutions

WB(1:100-1000), IHC-P(1:100-500)

Purification method

This antibody was purified via Protein A.

Long name

beta-Arrestin 1(Ser412) Antibody, HRP Conjugated

Specificity

This is a highly specific antibody against beta-Arrestin 1Ser412.

Crossreactive species details

Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.

Source

KLH conjugated synthetic phosphopeptide derived from human beta-Arrestin 1 around the phosphorylation site of Ser412

Synonyms

beta Arrestin 1 phospho S412; p-beta Arrestin 1 phospho S412; ARB 1; ARB1; ARR 1; ARR1; ARRB 1; ARRB1; Arrestin beta 1.

Storage conditions

Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% Gentamicin. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.

Properties

If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.HRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot.

Antigen background

Beta Arrestin 1 is a member of a family of proteins that are widely expressed but especially abundant in the central nervous system. Serving as an adaptor or scaffold molecule, beta Arrestin 1 is essential for mitogenic signaling. It mediates agonist dependent desensitization and internalization of G protein coupled receptors (GPCRs, e.g., beta 2 adrenergic receptor). After binding to their ligand and interacting with heterotrimeric G proteins, GPCRs are phosphorylated by G protein receptor kinases (GRKs) on serine residues. Beta Arrestin 1 has important roles in the cytoplasm and at the plasma membrane in the desensitization and internalization of G protein coupled receptors (GPCRs) and is increasingly appreciated to play an important role in the endocytosis and signaling of GPCRs. Beta Arrestin 1 in the cytosol is phosphorylated by ERK1 and 2 on serine 412 in a negative feedback mechanism and binds to the phosphorylated receptors at the plasma membrane. Serine 412 is then dephosphorylated and the GPCRs are internalized, leading to activation of the Ras, Raf, ERK1 and 2 signaling pathway.