beta-Arrestin 1(Ser412) Antibody, Cy5 Conjugated
- Size
- 0.1ml
- Catalog no.
- bs-3048R-Cy5
- Price
- 368 EUR
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Additional conjugation
Cy5.5
Tested applications
IF(IHC-P)
French translation
anticorps
Concentration
1ug per 1ul
Excitation Emission
625,650nm/670nm
Modification
Phosphorylation
Crossreactivity
Human, Mouse, Rat
Clone
Polyclonal antibody
Recommended dilutions
IF(IHC-P)(1:50-200)
Target Antigen
beta-Arrestin 1Ser412
Category
Conjugated Primary Antibodies
Host Organism
Rabbit (Oryctolagus cuniculus)
Purification method
This antibody was purified via Protein A.
Long name
beta-Arrestin 1(Ser412) Antibody, Cy5 Conjugated
Specificity
This is a highly specific antibody against beta-Arrestin 1Ser412.
Crossreactive species details
Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
Source
KLH conjugated synthetic phosphopeptide derived from human beta-Arrestin 1 around the phosphorylation site of Ser412
Synonyms
beta Arrestin 1 phospho S412; p-beta Arrestin 1 phospho S412; ARB 1; ARB1; ARR 1; ARR1; ARRB 1; ARRB1; Arrestin beta 1.
Storage conditions
Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
Properties
If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Cy5 antibodies are excited by the 650-nanometer wave of an argon laser and detected at a 670-nanometer captor.
Antigen background
Beta Arrestin 1 is a member of a family of proteins that are widely expressed but especially abundant in the central nervous system. Serving as an adaptor or scaffold molecule, beta Arrestin 1 is essential for mitogenic signaling. It mediates agonist dependent desensitization and internalization of G protein coupled receptors (GPCRs, e.g., beta 2 adrenergic receptor). After binding to their ligand and interacting with heterotrimeric G proteins, GPCRs are phosphorylated by G protein receptor kinases (GRKs) on serine residues. Beta Arrestin 1 has important roles in the cytoplasm and at the plasma membrane in the desensitization and internalization of G protein coupled receptors (GPCRs) and is increasingly appreciated to play an important role in the endocytosis and signaling of GPCRs. Beta Arrestin 1 in the cytosol is phosphorylated by ERK1 and 2 on serine 412 in a negative feedback mechanism and binds to the phosphorylated receptors at the plasma membrane. Serine 412 is then dephosphorylated and the GPCRs are internalized, leading to activation of the Ras, Raf, ERK1 and 2 signaling pathway.
